2019-02-19 19:42:072017-8-11 16:0:3
geneLength
1584
1581
Gene
Coordinates
3,129,530 3,131,113
3,129,530 → 3,131,113
The protein
[SW|Domains]
Nucleotide binding Domain (233240)
Nucleotide binding Domain (233–240)
Biological materials
Mutant
1A1005 ( ''pckA''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1005&Search=1A1005 BGSC]
1A996 ( ''pckA''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A996&Search=1A996 BGSC]
GP1147 (''pckA''::''neo''), available in [SW|Jrg Stlke]'s lab
BKE30560 ([[gene|pckA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGAAACCTTCCTTTAT, downstream forward: _UP4_TAAAAAACAAAAGCCAAGAG
BKK30560 ([[gene|pckA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGAAACCTTCCTTTAT, downstream forward: _UP4_TAAAAAACAAAAGCCAAGAG
1A1005 ( ''pckA''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1005&Search=1A1005 BGSC]
1A996 ( ''pckA''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A996&Search=1A996 BGSC]
GP1147 (''pckA''::''neo''), available in [SW|Jörg Stülke]'s lab
Biological materials
Expression vectors
pGP1753 (N-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP380]) (available in [SW|Jrg Stlke]'s lab) [pubmed|20933603]
pGP1762 (for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844], available in [SW|Jrg Stlke]'s lab)
pGP1763 (for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jrg Stlke]'s lab)
Biological materials
GFP fusion
GP1430 (spc, based on [SW|pGP1870]), available in the [SW|Stlke] lab
GP1430 (spc, based on [SW|pGP1870]), available in the [SW|Stülke] lab
Biological materials
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jrg Stlke]'s lab
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
Biological materials
FLAG-tag construct
GP1129 (spc, based on [SW|pGP1331]), available in [SW|Jrg Stlke]'s lab [pubmed|20933603]
GP1129 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab [pubmed|20933603]
labs
[SW|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France
Biological materials
Expression vector
pGP1753 (N-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP380]) (available in [SW|Jörg Stülke]'s lab) [pubmed|20933603]
pGP1762 (for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844], available in [SW|Jörg Stülke]'s lab)
pGP1763 (for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab)
Labs working on this gene/protein
[SW|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France