SubtiBank SubtiBank
Version comparison:

2019-02-19 19:42:072017-8-11 16:0:3

geneLength

1584

1581

Gene

Coordinates

3,129,530 3,131,113

3,129,530 → 3,131,113

The protein

[SW|Domains]

Nucleotide binding Domain (233240)

Nucleotide binding Domain (233–240)

Biological materials

Mutant

1A1005 ( ''pckA''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1005&Search=1A1005 BGSC]

1A996 ( ''pckA''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A996&Search=1A996 BGSC]

GP1147 (''pckA''::''neo''), available in [SW|Jrg Stlke]'s lab

BKE30560 ([[gene|pckA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGAAACCTTCCTTTAT, downstream forward: _UP4_TAAAAAACAAAAGCCAAGAG

BKK30560 ([[gene|pckA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGAAACCTTCCTTTAT, downstream forward: _UP4_TAAAAAACAAAAGCCAAGAG

1A1005 ( ''pckA''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1005&Search=1A1005 BGSC]

1A996 ( ''pckA''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A996&Search=1A996 BGSC]

GP1147 (''pckA''::''neo''), available in [SW|Jörg Stülke]'s lab

Biological materials

Expression vectors

pGP1753 (N-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP380]) (available in [SW|Jrg Stlke]'s lab) [pubmed|20933603]

pGP1762 (for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844], available in [SW|Jrg Stlke]'s lab)

pGP1763 (for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jrg Stlke]'s lab)

Biological materials

GFP fusion

GP1430 (spc, based on [SW|pGP1870]), available in the [SW|Stlke] lab

GP1430 (spc, based on [SW|pGP1870]), available in the [SW|Stülke] lab

Biological materials

two-hybrid system

''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jrg Stlke]'s lab

''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

Biological materials

FLAG-tag construct

GP1129 (spc, based on [SW|pGP1331]), available in [SW|Jrg Stlke]'s lab [pubmed|20933603]

GP1129 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab [pubmed|20933603]

labs

[SW|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France

Biological materials

Expression vector

pGP1753 (N-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP380]) (available in [SW|Jörg Stülke]'s lab) [pubmed|20933603]

pGP1762 (for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844], available in [SW|Jörg Stülke]'s lab)

pGP1763 (for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab)

Labs working on this gene/protein

[SW|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France